Regulatory

Part:BBa_K3669008:Design

Designed by: Paul Osuna-Kleist   Group: iGEM20_UC_Davis   (2020-10-27)


tetO7 operator sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Some plasmids only have 5 copies of tetO system, however 7 copies seems to lead to a better binding probability.


Source

Comes from E. Coli genome. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC546209/

References