Regulatory
Part:BBa_K3669008:Design
Designed by: Paul Osuna-Kleist Group: iGEM20_UC_Davis (2020-10-27)
tetO7 operator sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Some plasmids only have 5 copies of tetO system, however 7 copies seems to lead to a better binding probability.
Source
Comes from E. Coli genome. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC546209/